Difference between revisions of "Part:BBa J119390"

Revision as of 16:08, 29 April 2016

Collection of RBSs from N6 rClone Red Library

Oligonucleotides that encoded RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red rClone Red. to produce a library of 4096 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.

Sequence and Features