Difference between revisions of "Help:2016 InterLab Measurement Kit"
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<p>Before using your competent cells in an experiment, use the <b>Competent Cell Test Kit</b> (previously called, <b>Transformation Efficiency Kit</b>) to test the efficiency of your competent cells!</p>
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<p>New for 2016, we are providing teams with the <b>InterLab Measurement Kit</b>! The InterLab activity is completely optional and is not required for any team.</p>
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<p>The kit includes five vials of purified DNA from <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> (RFP construct) in plasmid backbone pSB1C3. Each vial contains DNA at a different concentration: 50pg/ul, 20pg/ul, 10pg/ul, 5pg/ul, 0.5pg/ul. Perform transformations with each of these to determine how efficient your competent cells are.</p>
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<p><i>Note:</i> Transformations performed with ligation products usually do not yield as many colonies because of the low DNA concentration in the ligation mixture. This means that you may see different results doing this test than you will at the end of the 3A Assembly protocol, or any other ligation.</p>
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<p>The kit includes 7 tubes (5 with plasmid DNA and 3 with standard reagents):
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<li>Test Device 1: J23101.B0034.E0040.B0015 in pSB1C3</li>
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<li>Test Device 2: J23106.B0034.E0040.B0015 in pSB1C3</li>
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<li>Test Device 3: J23117.B0034.E0040.B0015 in pSB1C3</li>
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<li>Positive Control Device: I20270 in pSB1C3</li>
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<li>Negative Control Device: R0040 in pSB1C3</li>
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<li>FITC Standard: two tubes with dried down FITC</li>
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<li>LUDOX: one tube with 30% colloidal silica suspended in 1mL of water</li>
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Revision as of 21:13, 12 April 2016

InterLab Measurement Kit

New for 2016, we are providing teams with the InterLab Measurement Kit! The InterLab activity is completely optional and is not required for any team.

The kit includes 7 tubes (5 with plasmid DNA and 3 with standard reagents):

  • Test Device 1: J23101.B0034.E0040.B0015 in pSB1C3
  • Test Device 2: J23106.B0034.E0040.B0015 in pSB1C3
  • Test Device 3: J23117.B0034.E0040.B0015 in pSB1C3
  • Positive Control Device: I20270 in pSB1C3
  • Negative Control Device: R0040 in pSB1C3
  • FITC Standard: two tubes with dried down FITC
  • LUDOX: one tube with 30% colloidal silica suspended in 1mL of water