Difference between revisions of "Help:2016 InterLab Measurement Kit"
(Created page with "{{HelpPage/MainLinks}} {{Help/3AAK_Style}} <html> <table width="100%"> <tr> <td class="headerstep"> Competent Cell Test Kit </td> <td rowspan=2> <img src="https://parts.igem.org/w...") |
|||
| Line 5: | Line 5: | ||
<tr> | <tr> | ||
<td class="headerstep"> | <td class="headerstep"> | ||
| − | + | InterLab Measurement Kit | |
</td> | </td> | ||
| + | |||
| + | <--! | ||
<td rowspan=2> | <td rowspan=2> | ||
<img src="https://static.igem.org/mediawiki/parts/0/0f/CompCellsTest_kit.png" style="height: 300px; border: 1px solid #000;"> | <img src="https://static.igem.org/mediawiki/parts/0/0f/CompCellsTest_kit.png" style="height: 300px; border: 1px solid #000;"> | ||
</td> | </td> | ||
</tr> | </tr> | ||
| + | --> | ||
| + | |||
<tr> | <tr> | ||
<td style="vertical-align: top; padding: 10px;"> | <td style="vertical-align: top; padding: 10px;"> | ||
Revision as of 20:24, 12 April 2016
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
| InterLab Measurement Kit | <--!
|
|
Before using your competent cells in an experiment, use the Competent Cell Test Kit (previously called, Transformation Efficiency Kit) to test the efficiency of your competent cells! The kit includes five vials of purified DNA from BBa_J04450 (RFP construct) in plasmid backbone pSB1C3. Each vial contains DNA at a different concentration: 50pg/ul, 20pg/ul, 10pg/ul, 5pg/ul, 0.5pg/ul. Perform transformations with each of these to determine how efficient your competent cells are. Note: Transformations performed with ligation products usually do not yield as many colonies because of the low DNA concentration in the ligation mixture. This means that you may see different results doing this test than you will at the end of the 3A Assembly protocol, or any other ligation. |