Difference between revisions of "Part:BBa J100252"

 
 
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<partinfo>BBa_J100252 short</partinfo>
 
<partinfo>BBa_J100252 short</partinfo>
  
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Started with tCloneTet (J119367) and used Golden Gate Assembly to remove GFP reporter and put transcriptional terminator riboswitch RS10shift (RS10s). RS10s was taken from the Wachsmuth et. al. 2013 NAR vol. 41 p. 2541. This riboswitch works and is responsive to theophylline (2.5mM).   
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Started with tCloneRed (J119361) and used Golden Gate Assembly to remove GFP reporter and put transcriptional terminator riboswitch RS10shift (RS10s). RS10s was taken from the Wachsmuth et. al. 2013 NAR vol. 41 p. 2541. This riboswitch works and is responsive to theophylline (2.5mM).   
  
 
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Latest revision as of 14:47, 23 March 2016

tCloneTet with RS10shift

Started with tCloneRed (J119361) and used Golden Gate Assembly to remove GFP reporter and put transcriptional terminator riboswitch RS10shift (RS10s). RS10s was taken from the Wachsmuth et. al. 2013 NAR vol. 41 p. 2541. This riboswitch works and is responsive to theophylline (2.5mM).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 773
    Illegal AgeI site found at 885
  • 1000
    COMPATIBLE WITH RFC[1000]