Difference between revisions of "Part:BBa M36142:Experience"
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===Applications of BBa_M36142===
 
===Applications of BBa_M36142===
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  This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher.  
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  This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher. When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made.  
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When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made.  
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Revision as of 04:25, 5 December 2015

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Please enter how you used this part and how it worked out.

Applications of BBa_M36142

This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher. When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made.


User Reviews

UNIQd0c0e9dc9ee751a2-partinfo-00000000-QINU UNIQd0c0e9dc9ee751a2-partinfo-00000001-QINU