Difference between revisions of "Part:BBa M36401:Design"

(→‎Design Notes)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
This part is derived from SAMT from Antirrhinus majus.
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The plasmid vector we used was adopted from an already existing plasmid backbone for E.Coli constructed by the company DNA 2.0. Since we used existing part within their parts catalog it allowed us to have a longer sequence for our genetic code. The original genetic sequence used for the project was taken from a pre-registered IGEM part which we took and then made slight alterations using Integrate DNA Technologies' codon optimization software to get rid of restriction sites and palindrome sequences.
 +
 
 +
This part is derived from SAMT from Antirrhinus majus.  
  
 
===References===
 
===References===
  
 
Part:BBa_J45001, who created the actual part that we adapted to fit our project.
 
Part:BBa_J45001, who created the actual part that we adapted to fit our project.
 +
https://www.idtdna.com/CodonOpt
 +
https://www.dna20.com/resources/genedesigner

Revision as of 23:25, 1 December 2015

Salicylic Acid to Methyl Salicylate Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 213
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 213
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 213
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A design considerations we had was the optimization of codons. We condon optimized this particular DNA sequence using IDT's Codon Optimzation Tool by changing its codons to match the most prevalent tRNAs. This way the protein will be more efficiently translated and make more of the desired product. This is the only relevant design change to this part.

Source

The plasmid vector we used was adopted from an already existing plasmid backbone for E.Coli constructed by the company DNA 2.0. Since we used existing part within their parts catalog it allowed us to have a longer sequence for our genetic code. The original genetic sequence used for the project was taken from a pre-registered IGEM part which we took and then made slight alterations using Integrate DNA Technologies' codon optimization software to get rid of restriction sites and palindrome sequences.

This part is derived from SAMT from Antirrhinus majus.

References

Part:BBa_J45001, who created the actual part that we adapted to fit our project. https://www.idtdna.com/CodonOpt https://www.dna20.com/resources/genedesigner