Difference between revisions of "Part:BBa K1728021"

Latest revision as of 16:34, 19 November 2015

Chemokine receptor 1 (CXCR1)

Chemokine receptor 1 (CXCR1) CXCR1 (also named with IL8RA and CD181) has high affinity in binding with IL8. CXCR1 belongs to the GPCR rhodopsin-like family and has seven-transmembrane domains, with the N-terminus exposed to the extracellular space and the C-terminus exposed to cytoplasm. Different from ordinary GPCR ligand binding sites, in which of CXCR1 locate on N-terminal and extracellular loop 3 of CXCR1. To improve heterologous expression of CXCR1 in yeast, we inserted two different signal peptide sequences upstream of the CXCR1 sequence. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 786
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 211
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 975

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 __NOTOC__
 <partinfo>BBa_K1728021 short</partinfo>
 Chemokine receptor 1 (CXCR1)
+CXCR1 (also named with IL8RA and CD181) has high affinity in binding with IL8. CXCR1 belongs to the GPCR rhodopsin-like family and has seven-transmembrane domains, with the N-terminus exposed to the extracellular space and the C-terminus exposed to cytoplasm. Different from ordinary GPCR ligand binding sites, in which of CXCR1 locate on N-terminal and extracellular loop 3 of CXCR1. To improve heterologous expression of CXCR1 in yeast, we inserted two different signal peptide sequences upstream of the CXCR1 sequence.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===