Difference between revisions of "Part:BBa K1728025"
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<partinfo>BBa_K1728025 short</partinfo> | <partinfo>BBa_K1728025 short</partinfo> | ||
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rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1) | rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1) | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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The result of pGal426 Lucy-rho-CXCR1 with BamHI and XhoI digestion. | The result of pGal426 Lucy-rho-CXCR1 with BamHI and XhoI digestion. | ||
Note: These two restriction enzyme site designed on insert sequence was used to check the ligation performance by enzyme digestion. Theoretically, there would be two bands. | Note: These two restriction enzyme site designed on insert sequence was used to check the ligation performance by enzyme digestion. Theoretically, there would be two bands. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 16:05, 19 November 2015
LUCY rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)
rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)
Usage and Biology
Construction of new yeast integrating plasmids
Plasmids cloning vectors that can “shuttle” DNA between yeast and bacteria we used is called pGAL426. Distinctly, it has different selection markers in yeast and bacteria. Lucy-rho-CXCR1 was inserted into this shuttle vector and finally transformed into FAR1△::KanMX-FUS1-GFP Gpa1 chimera strain.
The result of pGal426 Lucy-rho-CXCR1 with BamHI and XhoI digestion. Note: These two restriction enzyme site designed on insert sequence was used to check the ligation performance by enzyme digestion. Theoretically, there would be two bands. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 909
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 334
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 49
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1098
Illegal BsaI.rc site found at 63