Difference between revisions of "Part:BBa K1728025"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | ==Construction of new yeast integrating plasmids== | ||
| + | Plasmids cloning vectors that can “shuttle” DNA between yeast and bacteria we used is called pGAL426. Distinctly, it has different selection markers in yeast and bacteria. Lucy-rho-CXCR1 was inserted into this shuttle vector and finally transformed into FAR1△::KanMX-FUS1-GFP Gpa1 chimera strain. | ||
| + | https://static.igem.org/mediawiki/2015/3/3a/CGU-pGal426_Lucy-rho-CXCR1_with_BamHI_and_XhoI_digestion.jpg | ||
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| + | The result of pGal426 Lucy-rho-CXCR1 with BamHI and XhoI digestion. | ||
| + | Note: These two restriction enzyme site designed on insert sequence was used to check the ligation performance by enzyme digestion. Theoretically, there would be two bands. | ||
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Revision as of 15:53, 19 November 2015
LUCY rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)
rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 909
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 334
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 49
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1098
Illegal BsaI.rc site found at 63