Difference between revisions of "Part:BBa M36534:Design"

(→‎Design Notes)
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DNA 2.0 for general plasmid design including restriction sites, GAL1 inducible promoter, high copy number, and  
 
DNA 2.0 for general plasmid design including restriction sites, GAL1 inducible promoter, high copy number, and  
 +
 
Sequence for ACR 1 and 2 from yeastgenome website.  
 
Sequence for ACR 1 and 2 from yeastgenome website.  
 +
 
http://www.yeastgenome.org/locus/S000006403/sequence
 
http://www.yeastgenome.org/locus/S000006403/sequence
−
http://www.yeastgenome.org/locus/S000006404/sequence
 
−
 
  
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http://www.yeastgenome.org/locus/S000006404/sequence
  
 
===References===
 
===References===

Revision as of 02:44, 25 October 2015


Arsenate Bioremediation Actuator Plasmid Containing ACR 1 and ACR 2 Genes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 873
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ACR 2 was ordered first due its increased importance vs. ACR 1.


http://imageshack.com/a/img907/9338/NX12gL.png

Source

DNA 2.0 for general plasmid design including restriction sites, GAL1 inducible promoter, high copy number, and

Sequence for ACR 1 and 2 from yeastgenome website.

http://www.yeastgenome.org/locus/S000006403/sequence

http://www.yeastgenome.org/locus/S000006404/sequence

References