Difference between revisions of "Part:BBa K1321328:Design"

Latest revision as of 18:33, 2 October 2015

J-sRNA-331Bb

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 4541
Illegal EcoRI site found at 1297
Illegal SpeI site found at 1207
Illegal PstI site found at 1685
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1297
Illegal EcoRI site found at 4541
Illegal SpeI site found at 1207
Illegal PstI site found at 1685
Illegal NotI site found at 1678
Illegal NotI site found at 4547
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1297
Illegal EcoRI site found at 4541
Illegal XhoI site found at 1145
Illegal XhoI site found at 1151
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 4541
Illegal EcoRI site found at 1297
Illegal SpeI site found at 1207
Illegal PstI site found at 1685
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 4541
Illegal EcoRI site found at 1297
Illegal XbaI site found at 4556
Illegal SpeI site found at 1207
Illegal PstI site found at 1685
Illegal NgoMIV site found at 2860
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1198
Illegal BsaI.rc site found at 987

Revision as of 21:48, 20 October 2014 (view source) Mf1512 (Talk \| contribs) ← Older edit		Latest revision as of 18:33, 2 October 2015 (view source) Mf1512 (Talk \| contribs)
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	===Design Notes===		===Design Notes===
−	BBa_K1321302 was created by restricting BBa_K1033924 and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.	+