Difference between revisions of "Part:BBa J100235:Experience"
| Line 20: | Line 20: | ||
<center>[[File:araBAD1.png|600px|]]</center><br> | <center>[[File:araBAD1.png|600px|]]</center><br> | ||
| − | AraBAD promoter was tested using Golden Gate Assembly to insert the sequence into E. coli plasmids. Three petri dishes of E. coli colonies were prepared under three different conditions. The positive control contained a promoter known to cause production of red fluorescent protein. The negative control retained the original promoter of the plasmid, which produced green fluorescent protein. The experimental conditions tested the relative red fluorescence with respect to cell concentration in the presence and absence of arabinose of concentration 20µg/mL. Arabinose was the proposed inducer for the promoter. Each experimental condition shown in the graph was run in triplicate using a Synergy scanner to measure fluorescence. | + | AraBAD promoter was tested using Golden Gate Assembly to insert the sequence into ''E. coli'' plasmids. Three petri dishes of ''E. coli'' colonies were prepared under three different conditions. The positive control contained a promoter known to cause production of red fluorescent protein. The negative control retained the original promoter of the plasmid, which produced green fluorescent protein. The experimental conditions tested the relative red fluorescence with respect to cell concentration in the presence and absence of arabinose of concentration 20µg/mL. Arabinose was the proposed inducer for the promoter. Each experimental condition shown in the graph was run in triplicate using a Synergy scanner to measure fluorescence. |
Latest revision as of 19:27, 1 October 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J100235
User Reviews
UNIQ4ab8ac0e11eeb932-partinfo-00000000-QINU UNIQ4ab8ac0e11eeb932-partinfo-00000001-QINU
AraBAD promoter was tested using Golden Gate Assembly to insert the sequence into E. coli plasmids. Three petri dishes of E. coli colonies were prepared under three different conditions. The positive control contained a promoter known to cause production of red fluorescent protein. The negative control retained the original promoter of the plasmid, which produced green fluorescent protein. The experimental conditions tested the relative red fluorescence with respect to cell concentration in the presence and absence of arabinose of concentration 20µg/mL. Arabinose was the proposed inducer for the promoter. Each experimental condition shown in the graph was run in triplicate using a Synergy scanner to measure fluorescence.