Difference between revisions of "Part:BBa K1675021:Design"
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===References=== | ===References=== | ||
| + | ［1］ Le Y, Chen H, Zagursky R, et al. Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR[J]. DNA research, 2013: dst016. | ||
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| + | ［2］ Pai J C, Entzminger K C, Maynard J A. Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli[J]. Analytical biochemistry, 2012, 421(2): 640-648. | ||
Latest revision as of 00:09, 28 September 2015
P-atp2 mutant 705-B0034-lacz alpha
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.
Source
Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.
The natural P-atp2 is from Corynebacterium glutamicum.
Lacz alpha is from https://parts.igem.org/wiki/index.php?title=Part:BBa_I732006
References
［1］ Le Y, Chen H, Zagursky R, et al. Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR[J]. DNA research, 2013: dst016.
［2］ Pai J C, Entzminger K C, Maynard J A. Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli[J]. Analytical biochemistry, 2012, 421(2): 640-648.