Difference between revisions of "Part:BBa K1675009"

 
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<partinfo>BBa_K1675009 short</partinfo>
 
<partinfo>BBa_K1675009 short</partinfo>
  
 
Uses an acid induced promoter asr(BBa_K1170000), strong RBS(BBa_B0034),alkaline substance producing gene gadA coding region(BBa_K1675003).Will produce alkali under acidic environment and neutralize the external pH.
 
Uses an acid induced promoter asr(BBa_K1170000), strong RBS(BBa_B0034),alkaline substance producing gene gadA coding region(BBa_K1675003).Will produce alkali under acidic environment and neutralize the external pH.
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[[File:fermentation_1.png]]
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Fig.1 The final fermentation result of the basic regulation system under acidic stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.
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[[File:fermentation_2.png]]
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Fig.2 The growth curve of E.coli with basic regulation circuit under acidic stress.
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[[File:fermentation_3.png]]
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Fig.3 The final fermentation result of the basic regulation system under alkaline stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.
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[[File:fermentation_4.png]]
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Fig.4 The growth curve of E.coli with basic regulation circuit under alkaline stress.
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<!-- Add more about the biology of this part here

Revision as of 23:19, 27 September 2015

a device to produce alkali and neutralize the external acidic environment

Uses an acid induced promoter asr(BBa_K1170000), strong RBS(BBa_B0034),alkaline substance producing gene gadA coding region(BBa_K1675003).Will produce alkali under acidic environment and neutralize the external pH.

Fermentation 1.png

Fig.1 The final fermentation result of the basic regulation system under acidic stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.

Fermentation 2.png

Fig.2 The growth curve of E.coli with basic regulation circuit under acidic stress.

Fermentation 3.png

Fig.3 The final fermentation result of the basic regulation system under alkaline stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.

Fermentation 4.png

Fig.4 The growth curve of E.coli with basic regulation circuit under alkaline stress.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 844
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]