Difference between revisions of "Part:BBa K1689016:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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dCas9 fusion protein coding sequence is too long, so you'd better amplify it separately.
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The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
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+
−
 
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===Source===
 
===Source===

Revision as of 16:08, 27 September 2015


dCas9-F[1,2]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is very long. If you want to amplify only part of it (eg. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.

Source

E.coli

References