Difference between revisions of "Part:BBa K1689019:Design"
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Revision as of 15:36, 27 September 2015
delta alpha-dCas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4156
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 6435 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence of this fusion protein is very long. If you want to amplify only part of it (i.e. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
Source
E.coli