Difference between revisions of "Part:BBa K1689019:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The coding sequence of this fusion protein is too long, you'd better amplify it separately.
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The coding sequence of this fusion protein is very long. If you want to amplify only part of it (i.e. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.
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+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 15:34, 27 September 2015

delta alpha-dCas9



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 6435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is very long. If you want to amplify only part of it (i.e. dCas9 only), you'd better dissect the part you need into several segments, amplify them separately, and rejoin them using Gibson Assembly.

Source

E.coli

References