Difference between revisions of "Part:BBa K1689020:Design"
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Revision as of 15:19, 27 September 2015
dCas9-delta omega
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1150
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 3429 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence of this fusion protein is too long, you'd better dissect it into several segments and amplify it separately.
Source
E.coli