Difference between revisions of "Part:BBa K1741013"

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<partinfo>BBa_K1741013 short</partinfo>
 
<partinfo>BBa_K1741013 short</partinfo>
  
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A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.
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A hairpin introduced to 5’UTR slightly lowers transcription/translation. We are planning to extended it to use it as a thermometer, as for now we do not see temperature dependence.
  
 
https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg
 
https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg
  
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This is the potential hairpin sequence.
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This is the potential hairpin structure.
  
  

Revision as of 08:06, 27 September 2015

sfGFP under proC promoter with a hairpin in 5'UTR

A hairpin introduced to 5’UTR slightly lowers transcription/translation. We are planning to extended it to use it as a thermometer, as for now we do not see temperature dependence.

UAMpoznanproChairpin.jpg

This is the potential hairpin structure.


Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairpin to stop the translation in 23° C.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 232