Difference between revisions of "Part:BBa K1741013"

Revision as of 07:33, 27 September 2015

sfGFP under proC promoter with a hairpin in 5'UTR

A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.

This is the potential hairpin sequence.

Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 232

@@ Line 5: / Line 5: @@
 https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg
+This is the potential hairpin sequence.
+===Results===
+Legend:
+proC [[https://parts.igem.org/Part:BBa_K1741012  BBa_K1741012]]
+proC with a hairpin in 5'UTR [[https://parts.igem.org/Part:BBa_K1741013  BBa_K1741013]]
+proD [[https://parts.igem.org/Part:BBa_K1741014  BBa_K1741014]]
+https://static.igem.org/mediawiki/parts/3/35/Teamuamprocdiffsugarsgraph.png
+https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png
+https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png
+The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
+We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.