Difference between revisions of "Part:BBa K1741013"
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A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.
 
A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.
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https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:32, 27 September 2015

sfGFP under proC promoter with a hairpin in 5'UTR

A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.

UAMpoznanproChairpin.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 232