Difference between revisions of "Part:BBa K1741012"
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proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future. Expression is higher than of inducible promoters. | proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future. Expression is higher than of inducible promoters. | ||
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===Results=== | ===Results=== | ||
Revision as of 07:29, 27 September 2015
sfGFP under proC promoter
Design
proC moderately strong constitutive promoter (cf. Davis 2010) seems to reflect growth rate of E. coli bacterial strains on different media, and potentially can be a measure of protein biosynthesis rate, to be determined in future. Expression is higher than of inducible promoters.
Results
Legend:
proC [BBa_K1741012]
proC with a hairpin in 5'UTR [BBa_K1741013]
proD [BBa_K1741014]
The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 201