Difference between revisions of "Part:BBa K1741003"

Revision as of 07:23, 27 September 2015

sfGFP under melibiose promoter

Design

Chromosomal copy of MelR transcription factor is sufficient to activate the melibiose MelAB promoter copied from E. coli K12 chromosome, as well as MelWT which has a Pst1 site removed, when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than that of arabinose or rhamnose induced promoters.

Legend:

MelS [BBa_K1741004]

MelWT [BBa_K1741003]

Results

Legend:

MelS [BBa_K1741004]

MelWT [BBa_K1741003]

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We also checked the tightness of our promoters.

All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 154
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 206

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1741003 short</partinfo>
-==Design==
+===Design===
 Chromosomal copy of MelR transcription factor is sufficient to activate the melibiose MelAB promoter copied from E. coli K12 chromosome, as well as MelWT which has a Pst1 site removed, when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than that of arabinose or rhamnose induced promoters.