Difference between revisions of "Part:BBa K1741002"

Revision as of 07:17, 27 September 2015

sfGFP under a short arabinose promoter s2 without O1 and O2

This, shortened version of araBAD promoter (BBa_K1481002) does not contain O1 and O2 regions, but still contains the CRP binding site.

Design

Legend

AraWT - [BBa_K1481002]

Arashort1 - [BBa_1471000]

Arashort2 - [BBa_1471002]

For the design of our modified arabinose promoters we used the BioBrick created by our fellow iGEM team - PoznanBioInf. Their promoter was used as a control for out other arabinose promoters. We shortened the sequence of the original promoter by removing lengthy AraC part (we called that construct Arashort1) or removing AraC, O1 and O2 regions (we called that construct Arashort2).

Results

Legend

AraWT - [BBa_K1481002]

Arashort1 - [BBa_1471000]

Arashort2 - [BBa_1471002]

The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

All of our arabinose promoters are induced by the arabinose and repressed by the glucose. Arashort1 works better than AraWT. On the other hand, Arashort2 is slightly weaker when compared to Arashort1, but still stronger than AraWT. Probably O1 and O2 regions that were removed in Arashort2 are necessary for the best promoter's performance.

We also checked the tightness of our promoters.

All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.

Sequence and Features