Difference between revisions of "Part:BBa K1741007"

Revision as of 06:00, 27 September 2015

sfGFP under xylose promoter xylA (xylWT)

SfGFP under xylose promoter xylA (wild type).

=Design

Legend:

XylWT (XylA) [BBa_K1741007]

XylA1 [BBa_K1741008]

XylS [BBa_K1741009]

XylF [BBa_K1741010]

Genes involved in catabolism of xylose in E.coli are organized into two transcription units which are driven by two promoters - XylA and XylF. In order to increase the expression of a protein under our XylA1 promoter we have modified the 5'UTR of the XylWT promoter so it was similar to that of proD [BBa_K1741014], a strong constitutive promoter. The next step was to shorten the promoter by removing its XylF part while keeping the modified UTR. Finally we tested the XylF promoter upstream of sfGFP. XylS is the strongest xylose-induced promoter.

Characteristics and Results

Legend:

XylWT (XylA) [BBa_K1741007]

XylA1 [BBa_K1741008]

XylS [BBa_K1741009]

XylF [BBa_K1741010]

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

All our xylose promoters are induced by xylose and repressed by glucose. We also observed slight induction by arabinose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 153
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 408

@@ Line 3: / Line 3: @@
 SfGFP under xylose promoter xylA (wild type).
+===Design==
+Legend:
+XylWT (XylA) [[https://parts.igem.org/Part:BBa_K1741007 BBa_K1741007]]
+XylA1 [[https://parts.igem.org/Part:BBa_K1741008 BBa_K1741008]]
+XylS [[https://parts.igem.org/Part:BBa_K1741009 BBa_K1741009]]
+XylF [[https://parts.igem.org/Part:BBa_K1741010 BBa_K1741010]]
+Genes involved in catabolism of xylose in E.coli are organized into two transcription units which are driven by two promoters - XylA and XylF. In order to increase the expression of a protein under our XylA1 promoter we have modified the 5'UTR of the XylWT promoter so it was similar to that of proD [BBa_K1741014], a strong constitutive promoter. The next step was to shorten the promoter by removing its XylF part while keeping the modified UTR. Finally we tested the XylF promoter upstream of sfGFP. XylS is the strongest xylose-induced promoter.
 ===Characteristics and Results===
@@ Line 24: / Line 39: @@
 The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
-All our xylose promoters are induced by xyolse and repressed by glucose. Genes involved in catabolism of xylose in E.coli are organized into two transcription units which are driven by two promoters - XylA and XylF. In XylA1 we have modified the 5'UTR of the XylWT promoter so it was similar to that of proD [BBa_K1741014]a strong constitutive promoter in order to increase the expression of a protein under our promoter. The next step was to shorten the promoter by remaning its XylF part while keeping thw modified UTR. Finally we tested the XylF promoter upstream of sfGFP. XylS is the strongest xylose induced promoter
+All our xylose promoters are induced by xylose and repressed by glucose. We also observed slight induction by arabinose.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===