Difference between revisions of "Part:BBa K1741002"

 
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<partinfo>BBa_K1741002 short</partinfo>
 
<partinfo>BBa_K1741002 short</partinfo>
  
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This, shortened version of araBAD promoter does not contain O1 and O2 regions, but still contains the CRP binding site.  
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This, shortened version of araBAD promoter (BBa_K1481002) does not contain O1 and O2 regions, but still contains the CRP binding site.  
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===Characteristics and results===
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Legend
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AraWT - [[https://parts.igem.org/Part:BBa_K1481002 BBa_K1481002]]
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Arashort1 - [[https://parts.igem.org/Part:BBa_K1471000 BBa_1471000]]
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Arashort2 - [[https://parts.igem.org/Part:BBa_K1471002 BBa_1471002]]
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https://static.igem.org/mediawiki/parts/e/ec/Teamuampoznanarabinose2lbgraph.png
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https://static.igem.org/mediawiki/parts/5/56/Teamuampoznanaram9.png
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The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.
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All of our arabinose promoters are induced by the arabinose and repressed by the glucose. Arashort1 works better than AraWT. On the other hand, Arashort2 is slightly weaker when compared to Arashort1, but still stronger than AraWT. Probably O1 and O2 regions that were removed in Arashort2 are necessary for the best promoter's performance.
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We also checked the tightness of our promoters.
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All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.
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https://static.igem.org/mediawiki/parts/4/43/Teamuampoznanpromoterscomparisongrpah.png
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:30, 27 September 2015

sfGFP under a short arabinose promoter s2 without O1 and O2

This, shortened version of araBAD promoter (BBa_K1481002) does not contain O1 and O2 regions, but still contains the CRP binding site.

Characteristics and results

Legend

AraWT - [BBa_K1481002]

Arashort1 - [BBa_1471000]

Arashort2 - [BBa_1471002]

Teamuampoznanarabinose2lbgraph.png Teamuampoznanaram9.png

The sfGFP fluorescence [RFU] was measured using Tecan fluorometer.

All of our arabinose promoters are induced by the arabinose and repressed by the glucose. Arashort1 works better than AraWT. On the other hand, Arashort2 is slightly weaker when compared to Arashort1, but still stronger than AraWT. Probably O1 and O2 regions that were removed in Arashort2 are necessary for the best promoter's performance.


We also checked the tightness of our promoters.

All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose. Teamuampoznanpromoterscomparisongrpah.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 54
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 170