Difference between revisions of "Part:BBa K1741003"

Revision as of 05:21, 27 September 2015

sfGFP under melibiose promoter

MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.

Characteristics and Results

Legend:

MelS [Ba_K1741004]

MelWT [Ba_K1741003]

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 154
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 206

@@ Line 4: / Line 4: @@
 MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.
-===Results===
+===Characteristics and Results===
-Legend:<br>
+Legend:
 MelS [[https://parts.igem.org/Part:BBa_K1741004 Ba_K1741004]]
-<br>
 MelWT [[https://parts.igem.org/Part:BBa_K1741003 Ba_K1741003]]
-https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png
 https://static.igem.org/mediawiki/parts/2/2c/Teamuampoznanmelibioselbgraph.png
-https://static.igem.org/mediawiki/parts/a/a2/Teamuampoznandifferentpromotersm9minimal.png
+https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png
+The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.