Difference between revisions of "Part:BBa K1741003"

 
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<partinfo>BBa_K1741003 short</partinfo>
 
<partinfo>BBa_K1741003 short</partinfo>
  
 
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.  
 
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.  
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===Results===
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<br>
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Legend:<br>
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MelS BBa_K1741004
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<br>
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MelWT BBa_K1741003
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picture https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png
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picture https://static.igem.org/mediawiki/parts/2/2c/Teamuampoznanmelibioselbgraph.png
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picture https://static.igem.org/mediawiki/parts/a/a2/Teamuampoznandifferentpromotersm9minimal.png
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:44, 27 September 2015

sfGFP under melibiose promoter

MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.

Results



Legend:
MelS BBa_K1741004
MelWT BBa_K1741003

picture Teamuampoznanmelibiosem9graph.png picture Teamuampoznanmelibioselbgraph.png picture Teamuampoznandifferentpromotersm9minimal.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 206