Difference between revisions of "Part:BBa K1033250:Experience"
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===Applications of BBa_K1033250=== | ===Applications of BBa_K1033250=== | ||
| − | Utah State used this fluorescent protein in 2015 for a project engineering ''Lactococcus lactis'' to detect and fight against phi31 phage infection. It was not tested in ''L. lactis'' but did display function in ''E. coli.'' | + | Utah State used this fluorescent protein in their 2015 for a project engineering ''Lactococcus lactis'' to detect and fight against phi31 phage infection. It was not tested in ''L. lactis'' but did display function in ''E. coli'' when paired with three ''L. lactis'' promoters. (see Figure 1). |
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| + | https://static.igem.org/mediawiki/2015/0/0b/Utah_State_2015_MCherry_Fluorescence_Chart_Version_2.jpg | ||
| + | <p>Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 530/25 with emissions read at 590/35. cp8 from part BBa_K1820017, cp11 from part BBa_K1820018, and cp44 from part BBa_K1820019</p> | ||
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| + | <p> Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/ </p> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:50, 27 September 2015
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Applications of BBa_K1033250
Utah State used this fluorescent protein in their 2015 for a project engineering Lactococcus lactis to detect and fight against phi31 phage infection. It was not tested in L. lactis but did display function in E. coli when paired with three L. lactis promoters. (see Figure 1).
Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 530/25 with emissions read at 590/35. cp8 from part BBa_K1820017, cp11 from part BBa_K1820018, and cp44 from part BBa_K1820019
Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/
User Reviews
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