Difference between revisions of "Part:BBa K1613016"

Revision as of 20:10, 26 September 2015

It is a new part which can detect cadmium ions in liquid.

This part is designed to proceed cadmium detection in E.coli. ZinTp(YodA) K896008 is a promoter which is designed by NYMU in 2013. ZinTp (pYodA) is a promoter which expresses the downstream gene in the presence of cadmium ion. The activity of this promoter is specific affect by cadmium ion and won’t be induced by other ions like zinc, copper, cobalt, and nickel. Bacterial accommodation to moderate concentrations of cadmium is accompanied by transient activation of general stress proteins as well as a sustained induction of other proteins of hitherto unknown functions. One of the latter proteins was previously identified as the product of the Escherichia coli yodA ORF. The yodA ORF encodes 216 aa residues (the YodA protein) and the increased synthesis of YodA during cadmium stress was found probably to be a result of transcriptional activation from one single promoter upstream of the structural yodA gene. Analysis of a transcriptional gene fusion, ZinTp, yodA demonstrated that basal expression of yodA is low during exponential growth and expression is increased greater than 50-fold by addition of cadmium to growing cells. However, challenging cells with additional metals such as zinc, copper, cobalt and nickel did not increase the level of yodA expression. It is a part highly specific to the cadmium.The promoter can trigger the RFP. HSNU-TAIPEI used this part to detect cadmium ion in recycled oil.

Sequence and Features