Difference between revisions of "Part:BBa K1613002"

Revision as of 19:00, 26 September 2015

J22106:B0034

Introduce

J22106+B0034+E0040+B0015

We use recA promoter to control the reporter gene, because it can be activated by recA protein which is activated by ssDNA. So when E.coli,s sos response system works, the fluorescent gene can be expressed.

References

[1] Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1 [2] ROGER BRENT AND MARK PTASHNE Department ofBiochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 Contributed by Mark Ptashne, April 9, 1981. Mechanism of action of the lexA gene product

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1613002 short</partinfo>
-Construction intermediate
+=== Introduce ===
+[[File:Hsnu-taipei sos gfp.png|thumb| 500px |J22106+B0034+E0040+B0015  ]]
+We use recA promoter to control the reporter gene, because it can be activated by recA protein which is activated by ssDNA. So when E.coli,s sos response system works, the fluorescent gene can be expressed.
+=== References ===
+[1] Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1
+[2]  ROGER BRENT AND MARK PTASHNE Department ofBiochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 Contributed by Mark Ptashne, April 9, 1981. Mechanism of action of the lexA gene product
 <!-- Add more about the biology of this part here