Difference between revisions of "Part:BBa K1613000"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1613000 short</partinfo> | <partinfo>BBa_K1613000 short</partinfo> | ||
| − | == Introduce == | + | === Introduce === |
[[File:Hsnu-taipei sos gfp.png|thumb| 500px |J22106+B0032+E0040+B0015 ]] | [[File:Hsnu-taipei sos gfp.png|thumb| 500px |J22106+B0032+E0040+B0015 ]] | ||
We use recA promoter to control the reporter gene, because it can be activated by recA protein which is activated by ssDNA. So when E.coli,s sos response system works, the fluorescent gene can be expressed. | We use recA promoter to control the reporter gene, because it can be activated by recA protein which is activated by ssDNA. So when E.coli,s sos response system works, the fluorescent gene can be expressed. | ||
| − | == References == | + | === References === |
[1] Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1 | [1] Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1 | ||
[2] ROGER BRENT AND MARK PTASHNE Department ofBiochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 Contributed by Mark Ptashne, April 9, 1981. Mechanism of action of the lexA gene product | [2] ROGER BRENT AND MARK PTASHNE Department ofBiochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 Contributed by Mark Ptashne, April 9, 1981. Mechanism of action of the lexA gene product | ||
Revision as of 18:58, 26 September 2015
J22106:B0032
Introduce
We use recA promoter to control the reporter gene, because it can be activated by recA protein which is activated by ssDNA. So when E.coli,s sos response system works, the fluorescent gene can be expressed.
References
[1] Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1 [2] ROGER BRENT AND MARK PTASHNE Department ofBiochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 Contributed by Mark Ptashne, April 9, 1981. Mechanism of action of the lexA gene product
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]