Difference between revisions of "Part:BBa K1783002:Design"
Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | This BioBrick was | + | This BioBrick was synthesized by IDT. After linear pSB1C3 was amplified by PCR, the BioBrick was inserted into the backbone using NEB's HiFi Gibson assembly mix. The complete construct was transformed into both <i>E.coli</i> K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing. |
===Source=== | ===Source=== | ||
− | Promoter, RBS, LVA tag from E. coli | + | Promoter, RBS, LVA tag from <i>E. coli</i> |
− | RFP from Discosoma striata | + | <br> |
+ | RFP from <i>Discosoma striata</i> | ||
===References=== | ===References=== |
Latest revision as of 15:18, 26 September 2015
Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 619
Illegal AgeI site found at 731 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This BioBrick was synthesized by IDT. After linear pSB1C3 was amplified by PCR, the BioBrick was inserted into the backbone using NEB's HiFi Gibson assembly mix. The complete construct was transformed into both E.coli K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing.
Source
Promoter, RBS, LVA tag from E. coli
RFP from Discosoma striata