Difference between revisions of "Part:BBa K1783003:Experience"

Latest revision as of 15:17, 26 September 2015

Applications of BBa_K1783003

Uncomplicated transformation. Expression of RFP may differ based on strain of E.coli. According to experimentation, DH5α strain may more optimally express the protein of interest than BL21 strain.

The below graph is testing the plating of this construct in DH5α:

From the Hok/Sok plating tests we see that the resistance is maintained over several days, which is in contrast to negative control which has no selection method and rapidly loses resistance between 48-72 hours.

The below graph is testing the fluorescence of this construct in DH5α:

Fluorescence test show that Hok/Sok maintains fluorescence over a long period of time. This is in contrast to traditional antibiotic selection where we observed a loss of fluorescence over the same period of time due to the acquisition of mutations in the RFP construct. The exact reason as to why Hok/Sok does not lead to a reduction in RFP fluorescence over time is yet untested.

User Reviews

UNIQ30b580eb59903953-partinfo-00000000-QINU UNIQ30b580eb59903953-partinfo-00000001-QINU

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jueykang

Hok/Sok was capable of maintaining a plasmid over a long period of time. Hok/Sok was also able to maintain fluorescence over the same period of time for unknown reasons.

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@@ Line 9: / Line 9: @@
 The below graph is testing the plating of this construct in DH5α:
-[[https://static.igem.org/mediawiki/2015/d/d0/UMDHSplate2.png]]
+https://static.igem.org/mediawiki/2015/d/d0/UMDHSplate2.png
+From the Hok/Sok plating tests we see that the resistance is maintained over several days, which is in contrast to negative control which has no selection method and rapidly loses resistance between 48-72 hours.
 The below graph is testing the fluorescence of this construct in DH5α:
-[[https://static.igem.org/mediawiki/2015/d/d5/FtestHSUMD.png]]
+https://static.igem.org/mediawiki/2015/d/d5/FtestHSUMD.png
+Fluorescence test show that Hok/Sok maintains fluorescence over a long period of time. This is in contrast to traditional antibiotic selection where we observed a loss of fluorescence over the same period of time due to the acquisition of mutations in the RFP construct. The exact reason as to why Hok/Sok does not lead to a reduction in RFP fluorescence over time is yet untested.
 ===User Reviews===
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 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K1783003 EndReviews</partinfo>
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_K1783003 AddReview 5</partinfo>
+<I>jueykang</I>
+|width='60%' valign='top'|
+Hok/Sok was capable of maintaining a plasmid over a long period of time. Hok/Sok was also able to maintain fluorescence over the same period of time for unknown reasons.
+|};