Difference between revisions of "Part:BBa K1783003"
 
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<partinfo>BBa_K1783003 short</partinfo>
 
<partinfo>BBa_K1783003 short</partinfo>
  
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Produces unstable RFP under a constitutive promoter. This construct carries the hok/sok toxin/antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.
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Produces unstable RFP under a constitutive promoter. This construct carries the hok/sok toxin/antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.
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LVA-tagged unstable RFP has a half life of 1 hour allowing real-time data observations.  
  
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===Usage and Biology===
 
===Usage and Biology===
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We used this to test the ability of Hok/Sok to maintain plasmids over a long period of time. Hok/Sok is used naturally in the cell to maintain the R1 plasmid. RFP is a natural fluorescence protein found from coral.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1783003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1783003 SequenceAndFeatures</partinfo>
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Hok/Sok is directly adjacent to the RFP construct. This may be modified in future work by adding an insert in between Hok/Sok and RFP in order to decouple possible promoter interference.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Latest revision as of 15:04, 26 September 2015

Unstable RFP under Hok/Sok Regulation

Produces unstable RFP under a constitutive promoter. This construct carries the hok/sok toxin/antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics. LVA-tagged unstable RFP has a half life of 1 hour allowing real-time data observations.

Usage and Biology

We used this to test the ability of Hok/Sok to maintain plasmids over a long period of time. Hok/Sok is used naturally in the cell to maintain the R1 plasmid. RFP is a natural fluorescence protein found from coral.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 587
    Illegal NheI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 1311
  • 1000
    COMPATIBLE WITH RFC[1000]

Hok/Sok is directly adjacent to the RFP construct. This may be modified in future work by adding an insert in between Hok/Sok and RFP in order to decouple possible promoter interference.