Difference between revisions of "Part:BBa K1709003:Experience"
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<p>To characterize the CheZ-GFP BioBrick, the fragment containing a RBS was cloned directly after a strong promotor (BBa_J23101). The first figure shows the gel right before ligation.<br/> | <p>To characterize the CheZ-GFP BioBrick, the fragment containing a RBS was cloned directly after a strong promotor (BBa_J23101). The first figure shows the gel right before ligation.<br/> | ||
The colonies were checked by restriction mapping using BcuI and PstI (results not shown). The DNA sequence was also confirmed by DNA sequencing. Results can be provided by email. The presence of colonies expressing GFP proves that the plasmid was designed and cloned correctly.<br/> | The colonies were checked by restriction mapping using BcuI and PstI (results not shown). The DNA sequence was also confirmed by DNA sequencing. Results can be provided by email. The presence of colonies expressing GFP proves that the plasmid was designed and cloned correctly.<br/> | ||
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Further DNA sequencing proved the correct sequence, as designed. </p> | Further DNA sequencing proved the correct sequence, as designed. </p> | ||
Revision as of 14:46, 26 September 2015
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Applications of BBa_K1709003
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UNIQ89c522f6a553e34c-partinfo-00000000-QINU
To characterize the CheZ-GFP BioBrick, the fragment containing a RBS was cloned directly after a strong promotor (BBa_J23101). The first figure shows the gel right before ligation.
The colonies were checked by restriction mapping using BcuI and PstI (results not shown). The DNA sequence was also confirmed by DNA sequencing. Results can be provided by email. The presence of colonies expressing GFP proves that the plasmid was designed and cloned correctly.
Further DNA sequencing proved the correct sequence, as designed.
Gel after purification. Lanes 2-5: insert (1400bp). Lane 6: linearized vector. Lanes 7-10 : insert. Lane 11: linearized vector
GFP is expressed in the cells. This confirms the correct construction of the BioBrick
Further characterization could be done by transforming the cheZ knockout Keio strain with this plasmid. These cells should then regain their possibility to swim.
UNIQ89c522f6a553e34c-partinfo-00000001-QINU