Difference between revisions of "Part:BBa K1783004:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly.
 
This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly.
−
Was codon optimized for E. coli production using IDT Codon Optimizer.
+
Codon optimized using IDT Codon Optimizer.
  
 
===Source===
 
===Source===

Latest revision as of 04:49, 26 September 2015


Epsilon Cyclase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
    Illegal BamHI site found at 1398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
    Illegal AgeI site found at 1030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1105


Design Notes

This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. Codon optimized using IDT Codon Optimizer.

Source

From genome of Arabidopsis thaliana.

References