Difference between revisions of "Part:BBa K1783002:Experience"

Revision as of 04:39, 26 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1783002

This composite BioBrick served as a visual reporter gene. The short half life enables this part to be used as a method to quantify protein production in a more precise manner than a regular RFP that aggregates in the cell. A microplate reader was used to report objective data about the production of this protein. As a result of the constitutive promoter and short temporal lifespan, this part was used as an effective assay of plasmid maintenance. Alongside, plating tests were used to verify the presence of the plasmid of interest in the E.coli samples. This additional assay provides more evidence to verify the effectiveness of this protein over many generations as a reporter of protein production.

User Reviews

UNIQf44f9050af742a57-partinfo-00000000-QINU UNIQf44f9050af742a57-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1783002===
-This composite BioBrick was used as a visual reporter gene. The brief half life enables this part to be used as a method to quantify protein production in a much more precise manner than a regular RFP that aggregates in the cell. A microplate reader was used to report objective data about the production of this protein. As a result of the constitutive promoter and short temporal lifespan, this part was used as an effective assay of plasmid maintenance where the transcription and translation of this RFP reflected the maintenance of a plasmid that contained this part. Alongside of this visual reporter, an additional plating test was used to verify the presence of the plasmid of interest in the E. coli samples. The results of our plating tests demonstrate a strong correlation between RFP production and number of cells that contain the PSB1C3 plasmid. This additional assay provides more evidence to verify the effectiveness of this protein over many generations as a reporter of protein production.
+This composite BioBrick served as a visual reporter gene. The short half life enables this part to be used as a method to quantify protein production in a more precise manner than a regular RFP that aggregates in the cell. A microplate reader was used to report objective data about the production of this protein. As a result of the constitutive promoter and short temporal lifespan, this part was used as an effective assay of plasmid maintenance. Alongside, plating tests were used to verify the presence of the plasmid of interest in the <i>E.coli</i> samples. This additional assay provides more evidence to verify the effectiveness of this protein over many generations as a reporter of protein production.
 ===User Reviews===