Difference between revisions of "Part:BBa K1783002:Design"

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===Design Notes===
 
===Design Notes===
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This BioBrick was created by DNA synthesis through IDT. The G-block of this part was cloned into a linearized PSB1C3 backbone with Gibson assembly. The BioBrick was transformed and into both E. coli K12 DH5 alpha and BL21. The plasmids were miniprepped and verified through sequencing.
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This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. The BioBrick was transformed into both <i>E.coli</i> K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing.
  
 
===Source===
 
===Source===

Revision as of 04:35, 26 September 2015

Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. The BioBrick was transformed into both E.coli K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing.

Source

Promoter, RBS, LVA tag from E. coli RFP from Discosoma striata

References