Difference between revisions of "Part:BBa K1783002"
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<partinfo>BBa_K1783002 short</partinfo>
 
<partinfo>BBa_K1783002 short</partinfo>
  
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Produces unstable RFP under a constitutive promoter. This mutant RFP originated from coral (Part:BBa_E1010). This Biobrick includes a LVA-ssrA degradation tag that decreases the lifespan of this protein in E. coli. This LVA tag is reported to degrade RFP at a rate of -0.018 per minute.  
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Produces unstable RFP under a constitutive promoter. This mutant RFP originated from coral (Part:BBa_E1010). This BioBrick includes a LVA-ssrA degradation tag that decreases the lifespan of this protein in <i>E. coli</i>. This LVA tag is reported to degrade RFP at a rate of -0.018 per minute.  
  
 
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Revision as of 04:31, 26 September 2015

Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)

Produces unstable RFP under a constitutive promoter. This mutant RFP originated from coral (Part:BBa_E1010). This BioBrick includes a LVA-ssrA degradation tag that decreases the lifespan of this protein in E. coli. This LVA tag is reported to degrade RFP at a rate of -0.018 per minute.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]