Difference between revisions of "Part:BBa K1645999:Experience"
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===Applications of BBa_K1645999===
 
===Applications of BBa_K1645999===
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In order to characterize this part we used Imaging Flow Cytometry to see expression of both Red and Green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB over night. Then they were fixed and diluted 10 times in 10% formalin buffer and then there were places in Imaging FLow Cytometry and they were excited by one laser set at 488nm for optimum range reading. In order to control for signal leakage between channels a compensation was created using each of BBa_I20260 and BBa_J04450 as single colour controls. Then the intensity plot of both colours was created.  
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We used Imaging Flow Cytometry to characterise this part, as it allowed us to visualise the expression of both red and green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB overnight. The cells were fixed and diluted 10 times in 10% formalin buffer before use in Imaging FLow Cytometry, where they were excited by a laser optimised for reading at 488nm. In order to control for signal leakage between channels, BBa_I20260 and BBa_J04450 were used as single colour controls. This intensity plot of both colours was created.
  
 
The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. [[Link title]]
 
The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. [[Link title]]

Revision as of 03:21, 26 September 2015

Waterloo-2015-BBa-k1645999.png

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Please enter how you used this part and how it worked out.

Applications of BBa_K1645999

We used Imaging Flow Cytometry to characterise this part, as it allowed us to visualise the expression of both red and green fluorescent proteins. This part was transformed in DH5-alpha and grown in LB overnight. The cells were fixed and diluted 10 times in 10% formalin buffer before use in Imaging FLow Cytometry, where they were excited by a laser optimised for reading at 488nm. In order to control for signal leakage between channels, BBa_I20260 and BBa_J04450 were used as single colour controls. This intensity plot of both colours was created.

The results show that BBa_K1645999 expresses both RFP and GFP in DH5-alpha. Link title

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UNIQ30265d9a9c253225-partinfo-00000000-QINU UNIQ30265d9a9c253225-partinfo-00000001-QINU