Difference between revisions of "Part:BBa K1613012:Design"
Vin0916vin (Talk | contribs) (→Design Notes) |
Vin0916vin (Talk | contribs) (→References) |
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===References=== | ===References=== | ||
| + | Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, et al. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National Academy of Sciences. 2013 March 26;110(13):5010-5. (2013 IGEM CUHK) | ||
Latest revision as of 03:15, 26 September 2015
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 642
Illegal AgeI site found at 754 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a QsrR Binding Site between promoter and RBS. Which can combine with Quinone-sensing and response Repressor(QsrR). And inhibit the transcription. While QsrR combines with BaP-1,6-Quinone, the QsrR will be activated and depart the Binding Site. That makes the transcription of downstream.
Source
Promoter, RBS, Terminator, and RFP are came from IGEM plate.
References
Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, et al. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National Academy of Sciences. 2013 March 26;110(13):5010-5. (2013 IGEM CUHK)