Difference between revisions of "Part:BBa K1645998"

 
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<partinfo>BBa_K1645998 short</partinfo>
 
<partinfo>BBa_K1645998 short</partinfo>
  
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This part contains the 20 nucleotides long sequence which is complimentary to the region within the LacI (BBa_R0010) promoter, the scaffold region which is responsible for the secondary structure of the SgRNA, and the terminator. A constitutive promoter can should be placed upstream of this SgRNA in order for it to get transcribed.The suggested promoter to be used for this part is the U6 promoter.  
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This part contains the 20 nucleotides long sequence which is complimentary to the region within the LacI (BBa_R0010) promoter, the scaffold region which is responsible for the secondary structure of the SgRNA, and the terminator. A constitutive promoter should be placed upstream of this sgRNA in order for it to get transcribed.The suggested promoter to be used for this part is the U6 promoter. This promoter was used upstream of this part for our purposes of the project.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:55, 26 September 2015

SgRNA targeting LacI promoter

This part contains the 20 nucleotides long sequence which is complimentary to the region within the LacI (BBa_R0010) promoter, the scaffold region which is responsible for the secondary structure of the SgRNA, and the terminator. A constitutive promoter should be placed upstream of this sgRNA in order for it to get transcribed.The suggested promoter to be used for this part is the U6 promoter. This promoter was used upstream of this part for our purposes of the project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]