Difference between revisions of "Part:BBa K392001:Experience"
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===User Reviews=== | ===User Reviews=== | ||
| − | Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used | + | Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used. |
[[Image:Team_Tuebingen_pENO2-RFP.png|400px]] | [[Image:Team_Tuebingen_pENO2-RFP.png|400px]] | ||
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Figure 1: Fluorescence pictures of cells expressing pENO2-RFP. The RFP is localising to the plasma membrane on the cells. | Figure 1: Fluorescence pictures of cells expressing pENO2-RFP. The RFP is localising to the plasma membrane on the cells. | ||
| + | |||
| + | We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry. The sequence we found was: | ||
| + | |||
| + | TTATTATTTGTATAGTTCATCCATGCCATGTGTAATCCCAGCAGCTGTTACAAACTCAAGAAGGACCATGTGGTCTCTCTTTTCGTTGGGATCTTTCGAAAGGGCAGATTGTGTGGACAGGTAATGGTTGTCTGGTAAAnGGACAGGGCCATCGCCAATTGGAGTATTTTGTTGATAATGGTCTGCTAGTTGAACGCTTCCATCTTCAATGTTGTGTCTAATTTTGAAGTTAACTTTGATTCCATTCTTTTGTTTGTCTGCCATGATGTATACATTGTGTGAGTTATAGTTGTATTCCAATTTGTGTCCAAgAATGTTTCCATCTTCTTTAAAATCAATACCTTTTAACTCGATTCTATTAaCAAGGGTATCACCTTCAAACTTGACTTCAGCACGTGTCTTGTAGTTCC | ||
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Revision as of 02:26, 26 September 2015
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Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used.
Figure 1: Fluorescence pictures of cells expressing pENO2-RFP. The RFP is localising to the plasma membrane on the cells.
We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry. The sequence we found was:
TTATTATTTGTATAGTTCATCCATGCCATGTGTAATCCCAGCAGCTGTTACAAACTCAAGAAGGACCATGTGGTCTCTCTTTTCGTTGGGATCTTTCGAAAGGGCAGATTGTGTGGACAGGTAATGGTTGTCTGGTAAAnGGACAGGGCCATCGCCAATTGGAGTATTTTGTTGATAATGGTCTGCTAGTTGAACGCTTCCATCTTCAATGTTGTGTCTAATTTTGAAGTTAACTTTGATTCCATTCTTTTGTTTGTCTGCCATGATGTATACATTGTGTGAGTTATAGTTGTATTCCAATTTGTGTCCAAgAATGTTTCCATCTTCTTTAAAATCAATACCTTTTAACTCGATTCTATTAaCAAGGGTATCACCTTCAAACTTGACTTCAGCACGTGTCTTGTAGTTCC
UNIQ5b1bdd4b4b67a4f5-partinfo-00000000-QINU UNIQ5b1bdd4b4b67a4f5-partinfo-00000001-QINU