Difference between revisions of "Part:BBa K1613012"
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| + | Fig.1:The Gel Electrophoresis picture of BBa_K1613012. The plasmid was cut by restriction enzymes EcoRI and SpeI. There are three bands in the picture: Plasmids, Backbones, and Parts. | ||
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Revision as of 02:16, 26 September 2015
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.
There is a QsrR Binding Site between promoter and RBS. Which can combine with Quinone-sensing and response Repressor(QsrR). And inhibit the transcription. While QsrR combines with BaP-1,6-Quinone, the QsrR will be activated and depart the Binding Site. That makes the transcription of downstream.
Gel Electrophoresis:
Fig.1:The Gel Electrophoresis picture of BBa_K1613012. The plasmid was cut by restriction enzymes EcoRI and SpeI. There are three bands in the picture: Plasmids, Backbones, and Parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 642
Illegal AgeI site found at 754 - 1000COMPATIBLE WITH RFC[1000]