Difference between revisions of "Part:BBa K1680017"

Revision as of 02:14, 26 September 2015

Cre-Dronpa Fusion

Protein coding region for a fusion construct of Cre Recombinase (Part:BBa_K1680007) and 145N Dronpa (Part:BBa_K1680006) with intermediate 6 amino acid linker (Part:BBa_K1680000). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light. Part is cloned in the pTUM104 (Part:BBa_K801004) and thereby galactose inducible.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1327
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 449
Illegal SapI site found at 1046

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1680017 short</partinfo>
-Protein coding region for a fusion construct of Cre Recombinase (BBa_K1680007) and 145N Dronpa (BBa_K1680006) with intermediate 6 amino acid linker (BBa_K1680000). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light.
+Protein coding region for a fusion construct of Cre Recombinase ([[Part:BBa_K1680007]]) and 145N Dronpa ([[Part:BBa_K1680006]]) with intermediate 6 amino acid linker ([[Part:BBa_K1680000]]). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light.
-Part is cloned in the pTUM104 (BBa_K801004) and thereby galactose inducible.
+Part is cloned in the pTUM104 ([[Part:BBa_K801004]]) and thereby galactose inducible.
 <!-- Add more about the biology of this part here