Difference between revisions of "Part:BBa K392001:Experience"
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===User Reviews=== | ===User Reviews=== | ||
Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used. We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry. | Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used. We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry. | ||
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[[Image:Team_Tuebingen_pENO2-RFP.png|400px]] | [[Image:Team_Tuebingen_pENO2-RFP.png|400px]] | ||
Revision as of 02:10, 26 September 2015
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Figure 1 shows the results for the pENO2-RFP construct. Surprisingly, the RFP is localized only at the membrane, even though RFP without membrane target sequence was used. We therefore sequenced the whole construct and saw that the sequence of the promoter did not correspond to the sequence given in the registry.
Figure 4: Fluorescence pictures of cells expressing pENO2-RFP. The RFP is localising to the plasma membrane on the cells.
UNIQ91faed571ae62e92-partinfo-00000000-QINU UNIQ91faed571ae62e92-partinfo-00000001-QINU