Difference between revisions of "Part:BBa K1613023"

Revision as of 02:05, 26 September 2015

B0015:I721001

This part is a ligation of B0015 and I721001---the former fragments of K1613017. The description below is from the information of K1613017.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 217

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1613023 short</partinfo>
-Construction intermediate
+This part is a ligation of B0015 and I721001---the former fragments of K1613017. The description below is from the information of K1613017.
+This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP.
+The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.
 <!-- Add more about the biology of this part here