Difference between revisions of "Part:BBa K1680007"

Revision as of 02:01, 26 September 2015

Cre recombinase

This part contains the protein coding sequence for the DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two loxP sites (BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.

Sequence and Features

This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore not contain a start and stop codon.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 437

Revision as of 01:02, 26 September 2015 (view source) KatharinaS (Talk \| contribs) (→‎Sequence and Features) ← Older edit		Revision as of 02:01, 26 September 2015 (view source) KatharinaS (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1680007 short</partinfo>		<partinfo>BBa_K1680007 short</partinfo>

−	This part contains the protein coding sequence for the DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two ~~loxp~~ sites ([[BBa_K1680005]]) and depending on their orientation cuts out or reverses the DNA sequence between them.	+	This part contains the protein coding sequence for the DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two loxP sites ([[BBa_K1680005]]) and depending on their orientation cuts out or reverses the DNA sequence between them.