Difference between revisions of "Part:BBa K1680021"
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| − | This part contains the protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa [[part:BBa_K1680006]] using a 6 amino acid Ser/Gly-linker and the scar (Ser/Thr) as spacer | + | This part contains the protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa [[part:BBa_K1680006]] using a 6 amino acid Ser/Gly-linker [[part:BBa_K1680000]]and the scar (Ser/Thr) as spacer. The construct has an N-terminal NLS sequence [[part:BBa_K1680004]]. In the expressed construct the two Dronpa domains should form multimers leading to an inactivated Cre recombinase that is reversibly activatable by Dronpa illumination (see Zhou et al 2012). |
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Revision as of 01:12, 26 September 2015
Dronpa caged Cre with NLS
This part contains the protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa part:BBa_K1680006 using a 6 amino acid Ser/Gly-linker part:BBa_K1680000and the scar (Ser/Thr) as spacer. The construct has an N-terminal NLS sequence part:BBa_K1680004. In the expressed construct the two Dronpa domains should form multimers leading to an inactivated Cre recombinase that is reversibly activatable by Dronpa illumination (see Zhou et al 2012).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 298
Illegal BglII site found at 2032 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1154
Illegal SapI site found at 704
Illegal SapI site found at 1751